Microbiology Guide: Other Micro Biology Media (BART, Paddle Testers)

Document ID

Document ID TE8862

Published Date

Published Date 06/06/2018
Question
Microbiology Guide: Other Micro Biology Media (BART, Paddle Testers)
Summary
Microbiology Guide: Media and Equipment Selection; Other Micro Biology Media (BART, Paddle Testers)
Answer
From: Microbiology Guide: Media and Equipment Selection
  • Bilogical Activity Reaction Test (BART)
    • Media Packaging Options and Description
      • APB-BART Acid Producing Bacteria pk 9 (Catalog Number/Order Code 2831409)
        • Bacterial strains cpable of acid production under reductive (oxygen dprived) conditions
      • ALGE-BART Blue Gree Algae Bacteria pk 9 (Catalog Number/Order Code 2432709), pk 27 (Catalog Number/Order Code 2432727)
        • Photosynthetic plant-like microorganisms; cholrophyceae, cyanobacteria, desmids, diatoms and euglenoids
      • DN-BART Denitrifying Bacteria pk 9 (Catalog Number/Order Code 269309)
        • Nitrate reducing bacterial strains
        • Measures bacteria called the denitrifying bacteria and which are able to reduce the nitrate to nitrogen gas.
        • This test is applicable to any waters where there is likely to be potential septic or organic contamination. The presence of denitrifiers would indicate a potential health risk due to either septic wastes or nitrates in the water.
        • Gas forms a foam of bubbles around the ball, usuallywithin three days. The presence of this foam by the end of day two is taken to be an indication of an aggressive population of denitrifying bacteria.
        • Absence of foam, regardless of any clouding of the water, indicates that the test is negative for the detection of denitrifying bacteria.
      • FLOR-BART Fluorescing Pseudomonads pk 9 (Catalog Number/Order Code 2432609)
        • Fluorescing pseudomonad strains and related bacteria
        • Pseudomonads are bacteria very well adapted to breaking down some chemicals such as jet fuel and solvents but also can infest recreational waters and cause conditions ranging from skin, eye, ear, and nose infections to pneumonia-like infections.
        • The infectious pseudomonads produce an ultraviolet fluorescence that is usually a pale blue color.
        • Presence for this test means that either a greenish-yellow or a pale blue glow is generated by the careful application of an ultraviolet light just below the ball. The degraders tend to generate the greenish-yellow glow while the health risk group generates the pale blue glow.
        • A negative indication occurs when the sample remains clear.
      • HAB-BART Heterotrophic Aerobic Bacteria pk 9 (Catalog Number/Order Code 2490409), pk 27 (Catalog Number/Order Code 2490427)
        • Broad spectrum of aerobic bacteria
        • Determines the amount of heterotrophic aerobic bacterial activities, most common application is wastewater, particularly those that are aerobic.
        • This test relies upon the ability of the heterotrophic aerobic bacteria to reduce a methylene blue dye. To add the methylene blue to the sample, the test vial once charged is simply placed upside down for 30 seconds to allow the blue color to develop.
        • A positive reaction is detected by the blue color becoming bleached (due to the activity of methylene blue reductase). Bleaching may begin at the base of the test vial or just below the ball. Note that a residual blue ring is likely to remain around the ball, but this does not mean heterotrophs are absent.
        • A negative indication occurs when there is an absence of the blue color becoming bleached. This test is used to detect slimes plugging, taste and odor, cloudiness and can also detect the amount of aerobic heterotrophic activity on hazardous waste sites.
      • IRB-BART Iron Related Bacteria pk 9 (Catalog Number/Order Code 2432309), pk 27 (Catalog Number/Order Code 2432327)
        • Iron-related bacterial populations oxidizing, reducing, and “accumulating” strains
      • NB-BART Nitrifying Bacteria pk 9 (Catalog Number/Order Code 2619407)
        • Ammonium oxidizing strains
        • Ammonium is a common product of the breakdown of organic nitrogen under anaerobic (oxygen-free) conditions. The nitrifying bacteria oxidize the ammonium to nitrate in soils and waters. This nitrate now becomes a hazard if it builds up in the water.
        • This test is a different form of BART test in that it is fixed length and involves the detection of nitrite as an indicator of the presence of nitrifying bacteria. Nitrate is not detected because the amounts can be transient where there is a considerable amount of denitrification.
        • A reaction cap is used to allow the detection of the nitrite after a five-day incubation period.
        • A positive reaction occurs when a coloring is produced in the reaction cap
        • A  negative indication occurs when no coloring is produced in the reaction cap.
      • POOL-BART Pool and Spa Bacteria pk 9 (Catalog Number/Order Code 2478409)
        • Problematic pool and spa bacterial; Pseudomonas strains
      • SLYM-BART Slime Forming Bacteria pk 9 (Catalog Number/Order Code 2432509), pk 27 (Catalog Number/Order Code 2432527)
        • Aerobic/Anaerobic slime producing bacteria: Pseudomonads
        • A positive reaction involves a cloudy reaction in the inner test vial often with thick gel-like rings around the ball.
        • A negative test remains clear.
      • SRB-BART Sulfate-Reducing Bacteria pk 9 (Catalog Number/Order Code 2432409), pk 27 (Catalog Number/Order Code 2432427)
        • Anaerobic and aerobic hydrogen sulfide generating bacterial strains
        • Positive test occurs when  there is a blackening either in the base cone of the inner test vial (80% of the time) or around the ball (20% of the time).
        • A negative indication occurs when there is an absence of blackening in the base cone of the inner test vial or around the ball.
        • The culture medium is specific for the sulfate-reducing bacteria, such as Desulfovibrio and Desulfotomaculum. This is a more specific test and specifically relates to corrosion problems, taste & odor problems ("rotten egg" odors), and blackened waters. Slimes rich in SRB tend to also be black in color.
      • Combination Pak: IRB, SRB, SLYM qty 3 (each) (Catalog Number/Order Code 2434809)
    • Application Information
Application Problems associated with Bacterial growth Recommended BARTS
Aquaculture Algal blooms can deplete Oxygen supplies and produce toxins ALG, NB
Cooling Tower and Heat Exchangers Spoilage of cooling tower waters. which necessitates costly dumping. Corrosion of equipment. IRB, SRB, SLYM, HAB, DN, NB
Drinking/Water well drilling Corrosion and plugging of wells. Masking of coliform bacteria. IRB, SRB, SLYM, HAB
Farms and Private Wells Corrosion and plugging of wells and distribution lines. Fluctuation of Iron and Magnesium levels. Nitrification levels of soil. IRB, SRB, SLYM, HAB, NB
Haxardous Waste Treatment Facilities Biofouling by anaerobic and aerobic bacteria, from extraction to treatment. IRB, SRB, SLYM, HAB
Municipal Water Treatment (DW & WW) Regrowth in distribution lines. Offensive taste and odor in potable water. Corrosion of pipes and fixtures. Biofouling and corrosion of wastewater treatment equipment. IRB, SRB, SLYM, FLOR, HAB, DN, NB
Petroleum: oild field drilling and refining Pluging of wells. Corrosion of pipes. IRB, SRB, SLYM
Pools and Spas Unsanitary conditions harbor disease POOL, HAB
Power plant utilities Biofouling. Corrosion and plugging of pipes, which redces efficiency. IRB, SRB, SLYM, HAB
Process Water (Manufacturing) Corrosion and plugging of pipes. Which reduces efficiency. IRB, SRB, SLYM, HAB, DN, NB
Pulp and Pater plants Pluggin of pipes, reduced capacity, Speckled, poor-quality paper products. IRB, SRB, SLYM, DN, NB
Water Treatment chemicals and conditioning Biofouling and corrosion of sooling tower, boiler and home water systems. IRB, SRB, SLYM, HAB
  • Dip Slides & Paddle Testers
    • Concentration range: Paddle testers measure in the range from 100 organisms per mL to 100,000 organisms per mL by comparison to a colony density chart.
    • Media Packing
      • Total Aerobic Bacteria/Yeast & Mold pk 10 (Catalog Number/Order Code 2610810)
        • Tryptone glucose extract agar with Triphenyl Tetrazolium Chloride (TTC) for non-slective growth of total aerobic bacteria
          • TTC positive is red colonies
        • Modified sabouraud agar with Rose Bengal for selective isolation of yeasts and molds.
          • Rose Bengal positive is pink
          • Fiamentous growth is positive for molds
      • Total Aerobic Bacteria/Total Coliform Red Bile Agar pk 10  (Catalog Number/Order Code 2610910)
        • Tryptone glucose extract agar with Triphenyl Tetrazolium Chloride (TTC) for non-slective growth of total aerobic bacteria
          • TTC positive is red colonies
        • Violet red bile agar for enumerating total coliforms
          • Fermenting lactose coliforms product pink colonies
          • Non-fermenter produce colorless gray colonies
      • Total Aerobic Bactria/Disinfection Control pk 10 (Catalog Number/Order Code 2619510)
        • Tryptone glucose extract agar with Triphenyl Tetrazolium Chloride (TTC) for non-slective growth of total aerobic bacteria
          • TTC positive is red colonies
        • D/E neutralizing agar neutralizes several antiseptic and disinfectant chemicals.  Neutralization allows growth of bacteria held in bacteriostasis.