Microbiology Guide: Membrane Filtration (MF) Media

Document ID

Document ID TE8842

Published Date

Published Date 06/06/2018
Question
Microbiology Guide: Membrane Filtration (MF) Media
Summary
Microbiology Guide: Media and Equipment Selection; Membrane Filtration (MF) Media
Answer
From: Microbiology Guide: Media and Equipment Selection

Membrane Filtration (MF) Media Selection Summary

Drinking Water and Beverages
Organism USEPA- Accepted (Y/N) Presumptive Media Confirmation Media
Total Coliforms Y m-Endo Broth PourRite Ampules Lauryl Tryptose (LT) Broth Tubes (Single-Strength) and Brilliant Green Bile Broth Tubes or Coli-Firm Kit
 
Total Coliforms N m-ColiBlue24® Broth PourRight Ampules No Confirmation Necessary
Fecal Coliforms Y m-Endo Broth PourRight Ampules EC Medium Tubes
E. Coli Y M-Endo Broth PourRight Ampules EC Medium with MUG Tubes or Nutrient Agar with MUG Tubes
E. Coli N m-ColiBlue24® Broth PourRight Ampules No Confirmation Necessary
Heterotrophic Bacteria N m-TGE Broth PourRight Ampules or m-TGE with TTC Indicator Broth PourRight Ampules No Confirmation Necessary
Yeast and Mold N m-Green YM Broth PourRight Ampules No Confirmation Necessary

Wastewater and Recreational Water
Organism USEPA- Accepted (Y/N) Presumptive Media Confirmation Media
Fecal Coliforms Y m-FC Broth PourRight Ampules or m-FC with Rosolic Acid Broth PourRight Ampules No Confirmation Necessary
E. Coli Y m-TEC Agar Tubes Urea Substrate

Media With Confirmation
  • m-Endo Media, Hach method 8074, Presumptive for Total and Fecal Coliform
    • For potable, nonpotable, reacreation and wastewater samples
      • Adapted from SM 9222 B and 9221 B.
    • Packaging Options
      • Glass Ampule (Catalog Number/Order Code 2373520)
      • Plastic Ampule (Catalog Number/Order Code 2373550)
      • 100mL bottle (Catalog Number/Order Code 2373542)
      • Agar plates, pk 15 (Catalog Number/Order Code 2811615)
    • Confirmation Necessary
      • Total Coliform: lauryl tryptose & brilliant green bile
      • Fecal Coliform: EC medium
      • Ecoli Coliform: EC medium with MUG
 
  • m-ColiBlue24®, Hach method 10029, Simulateous measurement for Total and Ecoli Coliform
    • For potable, nonpotable, reacreation and wastewater samples
      • USEPA Approved
    • Packaging Options
      • Glass Ampule (Catalog Number/Order Code 2608420)
      • Plastic Ampule (Catalog Number/Order Code 2608450)
      • 100ml bottle (Catalog Number/Order Code 2608442)
      • Agar plates, pk 15  (Catalog Number/Order Code 2805215)
    • Confirmation
      • Oxidase Reagent (Catalog Number/Order Code 2622500)
 
  • m-FC & m-FC/RA, Hach method 8074, Fecal Coliform
    • For potable, nonpotable, reacreation and wastewater samples
      • USEPA approved 9222 D
    • Packaging Options m-FC
      • Glass ampules (Catalog Number/Order Code 2373220)
      • Plastic ampule (Catalog Number/Order Code 2373250)
      • Agar plates, pk 15  (Catalog Number/Order Code 2811515)
    • Packaging Options m-FC/RA
      • Glass ampule (Catalog Number/Order Code 2428520)
      • Plastic ampule (Catalog Number/Order Code 2428550)
    • Confirmation
      • Total Coliform: lauryl tryptose & brilliant green bile
      • Fecal Coliform: EC medium
      • Ecoli Coliform: EC medium with MUG
 
  • m-TEC, Hach method 8367, Ecoli Coliform
    • For potable, nonpotable, reacreation and wastewater samples
      • USEPA accepted
    • Packaging Options
      • m-TEC agar tubes (Catalog Number/Order Code 2561106)
    • Confirmation
      • Phenol Red Sodium Salt (Catalog Number/Order Code 2563922)
      • Sulfuric acid 0.02N (Catalog Number/Order Code 20342)
      • Urea Reagent  (Catalog Number/Order Code 1123726)
 
  • Modified m-TEC, Hach method 8367, Ecoli Coliform
    • For potable, nonpotable, reacreation and wastewater samples
      • USEPA accepted
    • Packaging Options
      • Modified m-TEC agar plates (Catalog Number/Order Code 2811815)
    • Confirmation not necessary
 
  • Heterotropic Bacteria Media, Hach method 8242
    • For water and wastewater samples
    • m-HPC plastic ampules (Catalog Number/Order Code 2812450)
    • m-HPC agar plates (Catalog Number/Order Code 1430598)
    • m-TGE glass ampule (Catalog Number/Order Code 2373850)
    • m-TGE with TTC glass ampule (Catalog Number/Order Code 2428420)
    • m-TSB/USP glass ampule (Catalog Number/Order Code 2812650)
    • Peel Plate® Heterotrophic Plate Count Media (Catalog Number/Order Code PP-HPC-50)
 
  • Enterococci Bacteria Media, Hach method 1600
    • For potable, nonpotable, reacreation and wastewater samples
    • Packaging Options
      • M-EI agar plates (Catalog Number/Order Code 2811715)
    • Confirmation
      • Brain Heart Infusion Broth (BHIB) (Catalog Number/Order Code 2815534)
      • Brain Heart Infusion Agar Slant (BHIA)  (Catalog Number/Order Code 2405634)
 
  • Pseudomonas Bacteria Media, Hach method 8026
    • For potable, nonpotable, reacreation and wastewater samples
    • Pseudomonas plastic ampules (Catalog Number/Order Code 2812250)
Media Decontamination
  • Recommendation:
    • Open each petri dish and pipet in 2-3 mL of Clorox, (you can use a disposable plastic dropper).
    • Cap the petri dish and swirl the wet the whole membrane filter.  Let stand for 20 – 30 minutes.
    • After waiting time place ‘all’ Cloroxed petri dishes in a Contaminated plastic bag for disposal.  Tie off or knot the bag.
    • Write Cloroxed on the Contamination bag.  Place into normal trash container. If using large plastic petri dishes, the process is the same except using 5 mL of Clorox to each plate.