What is the difference between m-TEC and modified m-TEC procedures?

Document ID

Document ID TE866

Version

Version 2.0

Status

Status Published

Published Date

Published Date 27/05/2017
Question
What is the difference between m-TEC and modified m-TEC procedures?
Summary
m-TEC vs. modified m-TEC method
Answer
The m-TEC method detects E. coli in recreational fresh water samples with a two step process. First, membrane filters are incubated on m-TEC Agar for 2 hours at 35 °C to resuscitate injured organisms. The thermotolerant organisms are then selected by fermentation of lactose at an elevated temperature of 44.5 °C. The second step uses a substrate medium containing urea to distinguish urease-negative E. coli from other thermotolerant coliforms that hydrolyze urea. Yellow or yellow-brown urease-negative colonies are positive for E. coli. vised single-step method uses modified m-TEC Agar to detect E. coli in recreational fresh water samples. The modified m-TEC Agar contains an enzymatic substrate which is cleaved to produce colonies. Red or magenta colored colonies specifically represent the presence of E. coli. Confirmation steps are not required with this method.