Are there instructions for BOD Standard Solution (Catalog Number 1486610) using the manometric method?

Document ID

Document ID TE3239

Version

Version 2.0

Status

Status Published

Published Date

Published Date 27/05/2017
Question
Are there instructions for BOD Standard Solution (Catalog Number 1486610) using the manometric method?
Summary
BOD Standard Solution Instruction
Answer

Improving Manometric BOD Test and Apparatus Accuracy:
Occasional analysis of a standard BOD sample will assure the operator that the manometric apparatus is functioning properly and that the procedure is correct. A widely accepted BOD standard is a mixture of 150 mg/L of glucose and an equal amount of glutamic acid. A prepared standard solution is available from Hach as the Voluette Ampule Standard for BOD, Cat. No. 14866-10 which when diluted 1:20 gives the required concentration. A procedure for analyzing standard BOD samples follows:
     a. Prepare approximately 3 L (¾ gal) of oxygen-saturated water by shaking a partially filled container of distilled water for one minute. Add the contents of one BOD Nutrient Buffer Pillow (Catalog Number 1486198) invert several times to mix.
     b. Snap the neck off a Voluette Ampule Standard for BOD (Catalog Number 1486610) and pipet 7 mL of standard into a sample bottle.
     c. Add 133 mL of the nutrient buffer solution prepared in Step a and 15-mL seed. A 10 % by volume of seed in solution will result. (For more information concerning seedings of samples, refer to Section V Paragraph 5.3 of the Manometric Manual.)
     d. Follow the general procedure for the BOD test on the seed while performing the test on the sample.
     e. Perform a full-strength BOD test on the seed while performing the test on the sample.
     f. To determine the BOD result, refer to Section V Paragraph 5.3.2 of the Manometric Manual. Corrected BOD of the standard solution should be 200 ± 37 mg/L.