How should the work area be prepared for microbiological tests?

Document ID

Document ID TE2845

Version

Version 3.0

Status

Status Published

Published Date

Published Date 25/01/2018
Question
How should the work area be prepared for microbiological tests?
Summary
Microbiology work area preparation
Answer
Start the incubator while preparing other materials. Set the incubator to the temperature required by the specific procedure (usually 35 +- 0.5 deg C for total coliforms and enterococci and 44.5 +- 0.2 deg C for fecal coliforms). Disinfect the work bench with a germicidal cloth, dilute bleach solution, bactericidal spray or dilute iodine solution. Wash hands thoroughly with soap and water. Mark each sample container with the sample number, dilution, date and other necessary information. Avoid contaminating the inside of the sample container in any way. Use presterilized Whirl-Pak® bags or bottles for sample collection. If the sample has been disinfected, use bags or bottles that contain a dechlorinating agent.