How do I use PolySeed?

Document ID

Document ID TE181

Published Date

Published Date 27/05/2017
Question
How do I use PolySeed?
Summary
Polyseed Preparation for BOD
Answer
Polyseed is a blend of microbial cultures packaged in capsules for BOD analysis.  Polyseed is designed to provide a uniform standard for the degredation of both industrial and municipal wastes in BOD analysis.

Preparation:
1. Prepare the seed by adding one PolySeed capsule to 500 mL of dilution water and stir gently or aerate for one hour.
2. The dilution water must contain a nutrient buffer pillow as described in the test procedure

Seed Control:
The seed itself will consume some dissolved oxygen that must be measured and used in calculating a seed correction factor.
1. Run the seed as a normal sample, so that depletion is at least 2 mg/L DO.
2. Use the depletion as well as ratio of volume of seed in sample to seed in the control to correct for the oxygen demand from the seed.

Use:
1. For the dilution method, add 1 to 2 mL (amount varies) of seed directly to each BOD bottle when measuring samples or standards.
* Use 15 mL if using the BODTrak.

This is described in more detail in the test procedure for each method.