Can high hydrogen sulfide values in a sample interfere with the results for the SRB-BART testers?

Document ID

Document ID TE1759

Published Date

Published Date 27/05/2017
Question
Can high hydrogen sulfide values in a sample interfere with the results for the SRB-BART testers?
Summary
SRB-BART hydrogen sulfide interference
Answer
One major challenge in using the sulfide-reducing bacteria (SRB) tester is dealing with samples that naturally contain hydrogen sulfide. Once the hydrogen sulfide concentration gets above 3 mg/L then there can be a spontaneous reaction with black sulfides forming in the tester. At greater than 20 mg/L hydrogen sulfide this black iron sulfide driven reaction can be immediate. Samples that are considered to have residual hydrogen sulfide should be pre-treated to remove this gas from the sample to prevent early false positives. The easiest manner to drive off the gas is by vigorously aerating the sample for fifteen minutes prior to starting the SRB-BART test. A small aquarium pump should provide adequate flows to remove the hydrogen sulfide and a small cotton plug should be placed in the air line to reduce the possibility of contamination of the sample from the air. (Information based on Droycon, Inc Standard Methods available on their website at www.dbi.ca).