How does the Total Kjeldahl Nitrogen Method 8075 work?

Document ID

Document ID TE1715

Published Date

Published Date 27/05/2017
Question
How does the Total Kjeldahl Nitrogen Method 8075 work?
Summary
Summary of Total Kjeldahl Nitrogen Method 8075
Answer
The term Total Kjeldahl Nitrogen refers to the combination of ammonia and organic nitrogen. However, only the organic nitrogen compounds that are present as organically bound nitrogen in the trinegative state are determined in this test. Nitrogen in this form is converted into ammonium salts by the action of sulfuric acid and hydrogen peroxide. The ammonia is then analyzed by a modified Nessler method test. The measurement wavelength is 460 nm for spectrophotometers or 420 nm for colorimeters.