What is the purpose of (presence/absence) P/A Broth with MUG and how is it used?

Document ID

Document ID TE1006

Published Date

Published Date 27/05/2017
Question
What is the purpose of (presence/absence) P/A Broth with MUG and how is it used?
Summary
P/A Broth with MUG is used to simultaneously detect total coliform bacteria and E. coli.
Answer
P/A Broth with MUG allows simultaneous detection of total coliform bacteria and E. coli. In addition to the lactose and lauryl tryptose broths with bromcresol purple, this medium contains MUG reagent (4-methylumbelliferyl-beta-D-glucuronide). The MUG reagent produces a fluorogenic product when hydrolyzed by glucuronidase (an enzyme specific to E. coli). MUG detects non gas-producing (anaerogenic) strains of E. coli.and works well when competitive organisms are present. Combine 100 mL of sample and P/A Broth with MUG, incubate for 24 hours and check for a color change and fluorescence. A yellow color indicates the presence of total coliforms. To detect E. coli, examine samples under a long-wave ultraviolet (UV) light. Fluorescence indicates the presence of E. coli.